In Situ:
other:Article Title: Marker-independent imaging reveals a correlation of fibrotic and epigenetic alterations in endometriosis
Article Snippet: IF stains were imaged utilizing a Zeiss LSM 880 (Zeiss Microscopy GmbH).
Article Title: Distinct tissue niches direct lung immunopathology via CCL18 and CCL21 in severe COVID-19
Article Snippet: Toponome Image Cycler® MM3 MelTec GmbH & Co.KG Magdeburg, Germany Superfrost Plus Gold slides Fisher Scientific, Jena, Germany 11976299 Zeiss LSM 880 Zeiss MH560 cryotome ThermoFisher cover slides 24 x 60 mm Menzel-Gläser 10672-015 “press-to-seal” silicone sheets Life technologies P24745 Illumina NextSeq 500/550 Illumina Nextseq 500 Illumina Novaseq 6000 S1 Illumina Makro fluoreszenz-cuvette Msscientific ChromatographieHandel GmbH, Berlin, Germany FF-1FL-G-5 Ultramicroscope UM-0108 LaVision BioTec LifeCanvas SmartClear II Pro Cairn Research, Faversham, United Kingdom https://lifecanvastech.com/produ cts/smartclear-ii-pro/ Cork plates, 20mm Slee medical GmbH #30001001 Glas slides, 76 x 26, SuperFrost Plus Langenbrink #041300 Embedding media Paraplast Plus Roth X881.1 OCT Compound, Tissue Tek Charité AK 4583 SS Supplementary Table 1.
Microscopy:Article Title: Pak , a downstream gene of ecdysone signaling, determines left–right polarity in the Drosophila brain through neuronal cell chirality
Article Snippet: .. Finally, the samples were clarified with ethanol and methyl salicylate (Tokyo Chemical Industry) and observed under an LSM700 or LSM 880 microscope (Carl Zeiss). .. The following primary antibodies were used: mouse monoclonal anti-Fas2 (diluted to 1:50, DSHB), rat monoclonal anti-GFP (diluted to 1:500, Nacalai Tesque), rabbit monoclonal anti-RFP (diluted to 1:500, Nacalai Tesque), and mouse monoclonal anti-Slit (diluted to 1:50, DSHB) antibodies.
Electron Microscopy:Article Title: The Cullin3–Ring E3 ubiquitin ligase complex and USP14 regulate spastin-mediated microtubule severing and promotion of neurite outgrowth
Article Snippet: Next, the cells were incubated with the secondary antibodies (anti-mouse Alexa Fluor 488, 1:1000, Thermo Fisher Scientific, Cat# A-10680; anti-rabbit Alexa Fluor 555, 1:1000, Thermo Fisher Scientific, Cat# A-21428; and anti-rat Alexa Fluor 647, 1:1000, Abcam, Cat# ab150159) at room temperature for 1 hour in the dark. .. The cells were mounted with Fluro-Gell II containing DAPI (Electron Microscopy Sciences, Hatfield, PA, USA, Cat# 17985-50) and imaged via confocal microscopy with an LSM 880 (Zeiss AG, Oberkochen, Germany). .. The fluorescence intensity within the area of cells of interest was measured using ImageJ software (version 1.51, National Institutes of Health, Bethesda, MD, USA), and the average fluorescence intensity of each cell was analyzed.
Confocal Microscopy:Article Title: The Cullin3–Ring E3 ubiquitin ligase complex and USP14 regulate spastin-mediated microtubule severing and promotion of neurite outgrowth
Article Snippet: Next, the cells were incubated with the secondary antibodies (anti-mouse Alexa Fluor 488, 1:1000, Thermo Fisher Scientific, Cat# A-10680; anti-rabbit Alexa Fluor 555, 1:1000, Thermo Fisher Scientific, Cat# A-21428; and anti-rat Alexa Fluor 647, 1:1000, Abcam, Cat# ab150159) at room temperature for 1 hour in the dark. .. The cells were mounted with Fluro-Gell II containing DAPI (Electron Microscopy Sciences, Hatfield, PA, USA, Cat# 17985-50) and imaged via confocal microscopy with an LSM 880 (Zeiss AG, Oberkochen, Germany). .. The fluorescence intensity within the area of cells of interest was measured using ImageJ software (version 1.51, National Institutes of Health, Bethesda, MD, USA), and the average fluorescence intensity of each cell was analyzed.
Software:Article Title:
Article Snippet: .. Software and code Policy information about availability of computer code Data collection Zeiss LSM 880 Zeiss black software Leica software (LAS X) Confocal laser scanning microscope (TCS SP8; Leica) Olympus ScanR Image Acquisition Software ImageQuant LAS 4000 for western blot AriaMx Real-Time PCR instrument (G8830A) for qPCRs Illumina Hiseq 4000 sequencer for ChIP-seq Comet Assay IV software (Perceptive Instruments, UK) Data analysis ImageJ/Fiji software (version 2.1.0/1.53c) Prism (version 8.4.2, GraphPad) Olympus ScanR Image Analysis Software (Version 3.0.1) Spotfire software (version 7.0.1; TIBCO 7.11) Comet Assay IV software (Perceptive Instruments, UK) Agilent AriaMx software (version 1.71) DESEQ2 for statistical analysis Bowtie2, MACS2, HOMER, deepTools For manuscripts utilizing custom algorithms or software that are central to the research but not yet described in published literature, software must be made available to editors and reviewers. ..
Laser-Scanning Microscopy:Article Title:
Article Snippet: .. Software and code Policy information about availability of computer code Data collection Zeiss LSM 880 Zeiss black software Leica software (LAS X) Confocal laser scanning microscope (TCS SP8; Leica) Olympus ScanR Image Acquisition Software ImageQuant LAS 4000 for western blot AriaMx Real-Time PCR instrument (G8830A) for qPCRs Illumina Hiseq 4000 sequencer for ChIP-seq Comet Assay IV software (Perceptive Instruments, UK) Data analysis ImageJ/Fiji software (version 2.1.0/1.53c) Prism (version 8.4.2, GraphPad) Olympus ScanR Image Analysis Software (Version 3.0.1) Spotfire software (version 7.0.1; TIBCO 7.11) Comet Assay IV software (Perceptive Instruments, UK) Agilent AriaMx software (version 1.71) DESEQ2 for statistical analysis Bowtie2, MACS2, HOMER, deepTools For manuscripts utilizing custom algorithms or software that are central to the research but not yet described in published literature, software must be made available to editors and reviewers. ..
Western Blot:Article Title:
Article Snippet: .. Software and code Policy information about availability of computer code Data collection Zeiss LSM 880 Zeiss black software Leica software (LAS X) Confocal laser scanning microscope (TCS SP8; Leica) Olympus ScanR Image Acquisition Software ImageQuant LAS 4000 for western blot AriaMx Real-Time PCR instrument (G8830A) for qPCRs Illumina Hiseq 4000 sequencer for ChIP-seq Comet Assay IV software (Perceptive Instruments, UK) Data analysis ImageJ/Fiji software (version 2.1.0/1.53c) Prism (version 8.4.2, GraphPad) Olympus ScanR Image Analysis Software (Version 3.0.1) Spotfire software (version 7.0.1; TIBCO 7.11) Comet Assay IV software (Perceptive Instruments, UK) Agilent AriaMx software (version 1.71) DESEQ2 for statistical analysis Bowtie2, MACS2, HOMER, deepTools For manuscripts utilizing custom algorithms or software that are central to the research but not yet described in published literature, software must be made available to editors and reviewers. ..
Real-time Polymerase Chain Reaction:Article Title:
Article Snippet: .. Software and code Policy information about availability of computer code Data collection Zeiss LSM 880 Zeiss black software Leica software (LAS X) Confocal laser scanning microscope (TCS SP8; Leica) Olympus ScanR Image Acquisition Software ImageQuant LAS 4000 for western blot AriaMx Real-Time PCR instrument (G8830A) for qPCRs Illumina Hiseq 4000 sequencer for ChIP-seq Comet Assay IV software (Perceptive Instruments, UK) Data analysis ImageJ/Fiji software (version 2.1.0/1.53c) Prism (version 8.4.2, GraphPad) Olympus ScanR Image Analysis Software (Version 3.0.1) Spotfire software (version 7.0.1; TIBCO 7.11) Comet Assay IV software (Perceptive Instruments, UK) Agilent AriaMx software (version 1.71) DESEQ2 for statistical analysis Bowtie2, MACS2, HOMER, deepTools For manuscripts utilizing custom algorithms or software that are central to the research but not yet described in published literature, software must be made available to editors and reviewers. ..
Chromatin Immunoprecipitation:Article Title:
Article Snippet: .. Software and code Policy information about availability of computer code Data collection Zeiss LSM 880 Zeiss black software Leica software (LAS X) Confocal laser scanning microscope (TCS SP8; Leica) Olympus ScanR Image Acquisition Software ImageQuant LAS 4000 for western blot AriaMx Real-Time PCR instrument (G8830A) for qPCRs Illumina Hiseq 4000 sequencer for ChIP-seq Comet Assay IV software (Perceptive Instruments, UK) Data analysis ImageJ/Fiji software (version 2.1.0/1.53c) Prism (version 8.4.2, GraphPad) Olympus ScanR Image Analysis Software (Version 3.0.1) Spotfire software (version 7.0.1; TIBCO 7.11) Comet Assay IV software (Perceptive Instruments, UK) Agilent AriaMx software (version 1.71) DESEQ2 for statistical analysis Bowtie2, MACS2, HOMER, deepTools For manuscripts utilizing custom algorithms or software that are central to the research but not yet described in published literature, software must be made available to editors and reviewers. ..
Single Cell Gel Electrophoresis:Article Title:
Article Snippet: .. Software and code Policy information about availability of computer code Data collection Zeiss LSM 880 Zeiss black software Leica software (LAS X) Confocal laser scanning microscope (TCS SP8; Leica) Olympus ScanR Image Acquisition Software ImageQuant LAS 4000 for western blot AriaMx Real-Time PCR instrument (G8830A) for qPCRs Illumina Hiseq 4000 sequencer for ChIP-seq Comet Assay IV software (Perceptive Instruments, UK) Data analysis ImageJ/Fiji software (version 2.1.0/1.53c) Prism (version 8.4.2, GraphPad) Olympus ScanR Image Analysis Software (Version 3.0.1) Spotfire software (version 7.0.1; TIBCO 7.11) Comet Assay IV software (Perceptive Instruments, UK) Agilent AriaMx software (version 1.71) DESEQ2 for statistical analysis Bowtie2, MACS2, HOMER, deepTools For manuscripts utilizing custom algorithms or software that are central to the research but not yet described in published literature, software must be made available to editors and reviewers. ..
Fluorescence:
Imaging:
Staining:
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